The moving object trace detection based on sequence images is a critical technology of target recognition and track processing. The core of the motion trajectory detection is the contour character extraction of the moving object, however traditional algorithms only highlights partial characters of the contour because of the different factors. On the basis of extracting different characteristic information from traditional algorithms, the multi-template integration edge detection method of ant colony clustering was adopted to obtain the edge of moving object. The experimental results show that this method can obtain relatively complete characteristic information in the course of trace detection and can attain the trace of the object more effectively.